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A. (1984). Optical sectioning microscopy: cellular architecture in three dimensions. Ann. Rev. Biophys. , 13, 191-219 Agard, D. , Shaw, P. J. and Sedat, J. W. (1989). Fluorescence microscopy in three dimensions. In Taylor, D. L. and Wang, Y. L. (Eds), Fluorescence Microscopy of Living Cells in Culture: B. Quantitative Fluorescence Microscopy-Imaging and Spectroscopy. Academic Press, New York, pp. 353-377 Agard, D. A. and Sedat, J. W. (1983). Three-dimensional architecture of a polytene nucleus.
1985). VECM thus provides the biologist with a straightforward method of observing dynamic phenomena in living cytoplasm with great clarity, and of elucidating their molecular basis and pharmacological responses in suitable model systems. 7 VECM micrographs of short segments of squid giant axon microtubules gliding in dissociated axoplasm on a glass surface. Gliding behaviour, which required ATP and is in the axial direction, as indicated by the arrows, is shown by almost all the microtubules in the field .
10 SIT camera video images of a living cultured chick cardiac myotube microinjected with rhodamine-labelled oc-actinin. (A) I day after microinjection and (B) 25 h later. Note that the immature myofibril with closely spaced oc-actinin bands in A (arrows) has developed into a larger mature myofibril with oc-actinin stained Z bands spaced at the adult separation, while another myofibril on the right side of the myotube (arrowhead) has appeared de novo. Bar= 10 Jlm. , 1986a, Journal of Cell Biology, 102, 2053- 2066, by copyright permission of the Rockefeller University Press) [;1 Figure I.