By Martine Geraerts, Catherine M. Verfaillie (auth.), Ulrich Martin (eds.)

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Aranguren XL, McCue JD, Hendrickx B, Zhu XH, Du F, Chen E, Pelacho B, Penuelas I, Abizanda G, Uriz M, Frommer SA, Ross JJ, Schroeder BA, Seaborn MS, Adney JR, Hagenbrock J, Harris NH, Zhang Y, Zhang X, Nelson-Holte MH, Jiang Y, Billiau AD, Chen W, Prosper F, Verfaillie CM, Luttun A (2008) J Clin Invest 118:505 20 M. M. Verfaillie 75. Schwartz RE, Reyes M, Koodie L, Jiang Y, Blackstad M, Lund T, Lenvik T, Johnson S, Hu WS, Verfaillie CM (2002) J Clin Invest 109:1291 76. Aranguren XL, Luttun A, Clavel C, Moreno C, Abizanda G, Barajas MA, Pelacho B, Uriz M, Arana M, Echavarri A, Soriano M, Andreu EJ, Merino J, Garcia-Verdugo JM, Verfaillie CM, Prosper F (2007) Blood 109:2634 77.

Numerous highly specific mAb, fluorochromes, oligonucleotides and various other moieties have all been covalently linked to MicroBeads, thereby transferring additional biochemical and physical properties to them [5, 6]. The nano-sized iron-dextran particles confer several unique features on MACS Technology. MACS MicroBeads are biodegradable and do not alter cell function. Effects on the functional status of cells by magnetic labeling with MicroBeads are primarily dependent on the target cell surface antigen and on the degree of crosslinking by mAb or ligands conjugated to the MicroBeads, but not on the MicroBeads themselves.

1 Introduction.................................................................................................................. 2 Selective Culturing of Embryonic Stem Cells............................................................. 3 Isolation and Enrichment of ESCs Based on Surface Markers.................................... 4 Sorting of Cell Types Derived from Embryonic Stem Cells........................................ 5 Sorting Based on Genetically Modified Embryonic Stem Cells.................................

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