By Ronald M. Atlas

Atlas (biology and public healthiness, U. of Louisville) offers a few 2000 alphabetical entries that offer info on media which are used for environmental microbial analyses, masking the diversity of media had to domesticate the range of microorganisms which are culurable. each one access presents the names and synonyms of media, description of composition, directions for instruction, advertisement resources, makes use of, and safeguard cautions the place wanted. He additionally contains a short advent to the numerous elements of media and the first guidance equipment.

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Extra resources for Handbook of Media for Environmental Microbiology

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25g (NH4)2SO4 ..................................................................... 0L. Mix thoroughly. 0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Use: For the cultivation and maintenance of Alicyclobacillus acidoterrestris. 2g Agar solution ......................................................... 0mL Trace Elements Solution SL-6................................... 2g ZnSO4·7H2O.................................................................

Preparation of Medium: Prepare and dispense medium under 100% N2 gas atmosphere. 0mL. Mix thoroughly. Gently heat and bring to boiling. Boil for 5 min. Cool to room temperature while sparging with 100% N2. Add solid NaHCO3, NH4Cl, and Na2CO3. Mix thoroughly. Distribute into anaerobe tubes or bottles. Autoclave for 15 min at 15 psi pressure–121°C. 0mL vitamin solution. 0. 2g ZnSO4·7H2O................................................................. 1g Na2MoO4·2H2O..........................................................

Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically add sterile sodium sesquicarbonate solution. Mix thoroughly. 7. Pour into sterile Petri dishes or distribute into sterile tubes. 0L. Mix thoroughly. 0. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes. Use: For the cultivation of pectinolytic bacteria. 0L. Mix thoroughly.

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