By Ivan N. Rich (eds.)

This volumepresents updated equipment that permit basic stem cells from a number of assets to be remoted, cultured in vitro, detected and measured for particular functions. those functions diversity from these in simple, stem phone and veterinary learn to toxicology, mobile remedy and regenerative medication. there's a mild bias in the direction of the blood-forming process as extra is understood concerning the blood-forming or hematopoietic method than the other fundamental stem cellphone procedure. those certain houses and features are mentioned and tested, as a rule on the mobile point and intimately during this booklet. Written within the winning Methods in Molecular Biology sequence structure, chapters contain introductions to their respective themes, lists of the mandatory fabrics and reagents, step by step, conveniently reproducible protocols, and notes on troubleshooting and fending off recognized pitfalls.

Authoritative and available, Stem cellphone Protocols offers newbies with the basics essential to enhance new applied sciences worthy for simple and scientific examine sooner or later, and may reduction pros to find new methodologies to supply a much wider standpoint and an excellent larger scope for his or her personal research.

Show description

Read or Download Stem Cell Protocols PDF

Best methodology & statistics books

How to Research

Examine innovations to discover the data you wish This revised 3rd version offers necessary information on how to gather information, write proposals, and make shows. providing you step by step innovations for the best and centred examine at school or paintings tasks, the right way to examine comprises hands-on routines that construct up abilities and boost examine options helpful for any venture, in any box.

Teilhard's vision of the past: the making of a method

The Phenomenon of guy, by means of Pierre Teilhard de Chardin, has been characterised as metaphysics, poetry, and mysticism-virtually every thing other than what its writer claimed it was once: a in simple terms medical mmoir. Professor O'Connell right here follows up on a nest of clues, exposed first in an early unpublished essay, then within the sequence of essays contained mostly within the imaginative and prescient of the earlier.

Gene Transfer and Expression: A Laboratory Manual

A pragmatic guide of protocols for attaining expression of international genes in mammalian cells. It comprises a few very new options corresponding to PCR-based expression. the writer offers a theoretical creation to the protocols and compares the strengths and weaknesses.

The economic laws of scientific research

'Dr Kealey's courageous, unique and realized publication makes a strong case for his unpopular perspectives. It needs to supply pause to any open-minded scholar of technological know-how coverage. ' - R. C. O. Matthews 'Not when you consider that J. D. Bernal has a training British scientist challenged traditional arguments concerning the investment of technology so initially, and so powerfully.

Extra resources for Stem Cell Protocols

Example text

3. Alexa Fluor® 700-conjugated anti-mouse c-Kit (CD117) antibody. 4. Pacific blue (PB)-conjugated anti-mouse Sca-1 (Ly6A/E) antibody. 5. Phycoerythrin (PE)-conjugated (CD135) antibody. anti-mouse Flk2/Flt3 6. Fluorescein-5-isothiocyanate (FITC)-conjugated anti-mouse CD34 antibody. 7. Alexa Fluor® 647 anti-mouse CD150 (SLAM) antibody. 8. Hoechst 33342 is a DNA dye that can be pumped out by stem and progenitor cells. Stem/progenitor cells thus have a 24 Yi Liu et al. Hoechst low fluorescence in both the blue and red regions of the spectrum.

4 Stem Cell Marker Staining 27 1. Add CD34-FITC to lineage-depleted cells (main sample) at a concentration of 1:25. Incubate for 90 min at 4 °C on a rocker. Add 2 μL of CD34-FITC into Control 3 tube. 2. Incubate for 1 h, and add other markers for stem cell staining. The panel of markers and the corresponding concentrations used in the authors’ laboratory are Sca-1 (1:100), c-Kit (1:100), Flk2/ Flt3 (1:100), and CD150 (1:100). Streptavidin is also added to the cell suspension to bind biotinylated lineage antibodies (1:150).

1 mL using non-sterile tips. 8. Electronic cell counter or flow cytometer that can determine nucleated cell counts. 9. , hemocytometer, flow cytometer, viability counter). 10. Sterile, conical 15 and/or 50 mL tubes with screw lid. 11. Sterile, 5 mL tubes with snap on lid. 12. 5 mL Eppendorf conical vials. 2 Reagents 37 1. Dulbecco’s phosphate buffered saline (dPBS). 2. Iscove’s Modified Dulbecco’s Medium (IMDM). 3. 077, Axis-Shield; or Ficoll, GE Healthcare). 4. Cryopreservation medium: IMDM + 10 % fetal bovine serum (FBS) + dimethylsulfoxide (DMSO).

Download PDF sample

Rated 4.45 of 5 – based on 17 votes